How much pcr product to load on gel

Running the Gel Steps: Once all samples have been loaded, attach a lid to the rig, and attach the lid to the BioRad power supply NOTE: always make sure that the current is off or paused before inserting or removing a cords from the power supply.

Set the voltage to V and run the gel for about minutes you can set a timer on the machine itself by clicking on the clock logo. The run will stop when the time runs out. It is advisable to check up on the gel from time to time to make sure that it is proceeding normally.

Once centered, close the imager door and select Run Protocol. Modify the image as you see fit. Note that the program saves files as. For a simpler version which can be viewed from any computer, select snapshot, then save a common file type ex:. If you want a physical copy you can also print the image. Hitting the change contrast button allows manipulation of the brightness of the image, which may be helpful to clearly see faint bands.

By clicking Lanes and Bands, then Automatic, the program puts labeled lanes where it perceives them. Bands can similarly be applied to the image. KateElston - 07 Jun Contributors to this topic AurkoDasgupta, KateElston. Topic revision: r2 - - - Main.

The higher concentration, the less you need to add. Also, the bigger your well in width, the more you you need to add. For a PCR product that you are trying to clean up, I would load as much as you can get. It hard to say, it depend or how much amplification you have.

If you have a very good amplification, you can run ul of your PCR reaction, better if you load everything. Use again depend of the ladder you are using for example 5 ul of 0. And very important it depend the kit you are using to purify your DNA, take account of the specification in the kit. Hi, Thanks Means for a bigger well is sufficient? I generally just go ahead and run 10 ul per well 5 if I'm really trying not to use up my pcr product for a well comb in a mini gel.

The promega ladder that we use calls for 5 ul.